Preparation of drugs serous fluid for microscopic examination

Preparations for microscopic examination prepared as of sludge, and of fibrinous clots or clots and shreds. After settling of serous fluid for 1-2 hours a glass tube collected sediment centrifugation (as in examination of urine). When large amounts of fluid collected precipitate several centrifuge tubes (to 10), centrifuged for 5-10 minutes at 1500-3000 / min, after which all the precipitates obtained is poured into a test tube and centrifuged again.

The result is a concentrated precipitate, which is prepared from native preparations for microscopic examination. In the presence of fluid in the fibrinous clots and shreds in the analysis describe the number and volume. Convolution and scraps recovered from the liquid in a Petri dish, and then with a spatula, and a needle is cleaved from their pieces for the preparation of native products. If the parcel adheres to the glass, it is necessary to stretch the needle and spatula. Otherwise, it turns thick drug, not suitable for microscopic examination (cells it will be indistinguishable).

After microscopic examination of native specimens were stained with Romanovsky or Pappenheim no more 5 m. In the presence of serous fluid pus, clots and shreds of sediment smears are prepared and stained by Ziehl-Nelsenu and Gram.

During the cytological diagnosis you need to know, taken from the material under test, manner in which it was obtained, presumptive clinical diagnosis, as well as having information about, whether the patient was exposed to radiation therapy and chemotherapy, or other interventions, which could injure the serous membranes, and cause destructive and degenerative changes in the cells.