Preparation and investigation of ejaculate
Sperm (spermatozoonov) Ham and Leeuwenhoek discovered back in the 80s of the XVII century., but the development of the science spermatologii virtually began in the mid-20th century.
One of the modern social Problems spermatologii, requiring authorization by the proper organization of medical and preventive care - the fight against male infertility. According to available data, in 40 % cases of male infertility is the cause of sterile marriages. An important role in solving this problem is given clinical laboratory diagnostics. Timely implementation in practice of clinical laboratory diagnostics achievements in the field of spermatologii (research mobility with the identification of live sperm among fixed, morphological, Biochemical, biological research and other sperm.) It reveals, along with frequent cases of sperm pathology, previously diagnosed, such, as the relative infertility in immunological incompatibility, latent diseases, etc..
Treatment of male infertility It is effective in the presence of a permanent working contacts between clinical and laboratory professionals (Urologist, ginekologom, sometimes endocrinologist and geneticist), otherwise it may cause serious errors in diagnosis and treatment of patients. For Example, Late surgery for cryptorchidism gives only a cosmetic effect, as the fertilizing capacity can not be restored. When non-recognition of endocrine pathology wrong treatment leads to infertility, whereas timely appropriate treatment in similar cases, can lead to the full restoration of the fertilizing capacity of sperm.
A survey of spouses, applied over a barren marriage, It should begin with the study of the ejaculate or husband (in its rejection of the survey) with postcoital samples. Laboratory examination of ejaculate It makes it possible to obtain information on the status of the male reproductive system and its functions.
Getting ejaculate
Ejaculate - A liquid, released from the urethra during ejaculation one. Only with sperm ejaculate sperm can be called, or semen.
Get ejaculate through masturbation or with a special vibrating device in a separate room at lab. Collect material in a clean, dry glass or Teflon, desirable graded, dishes. For this purpose, you can use the measuring cylinders (volume 10 ml) or tubes deployed neck. In some cases, you can allow the delivery of semen to the laboratory of the house, but only in containers, prepared in the laboratory. Deliver ejaculate in the condom or getting it by interrupting intercourse inadmissible, as it leads to considerable errors and distortions of the results.
It is recommended to receive the ejaculate for the study after 3-5 days of sexual abstinence. At this time should not drink alcohol, taking medications, smoke, sauna, and also to massage the prostate and seminal vesicles.
The resulting ejaculate (in laboratory or at home) you must immediately, preventing cooling, placed in thermostat, since temperature drop may change sperm motility.
Research ejaculate It is usually carried out one hour after ejaculation. Prior to the study should be noted during ejaculate liquefaction. Normally, immediately after ejaculation semen coagulate under the action of the enzyme coagulase, contained in the seminal vesicles. Liquefaction ejaculate comes after 5-30 minutes after ejaculation depending on the activity of proteolytic enzymes, contained in prostatic secretions and spermatozoa.
