Errors laboratory diagnostics in the study of pathogenic protozoa

In the study of smears and thick drops of blood for malaria is sometimes mistaken platelets. A single platelet, lying on the erythrocyte, It can be taken as merozoite, a cluster of them - for morula. Platelets differ from merozoites its color and character of the cytoplasm: Platelets have a pale pink color, fine, rosier grain, and merozoite consists of a compact nucleus of red and blue cytoplasm.

In blood smears sometimes found extraneous organisms, got outside - mushrooms, seaweed, protozoa etc.. Particular similarities to malaria parasites have some kinds of mushrooms round or oval-shaped with a compact form in the center, similar to the core. Scraps of white blood cells, Education with harnesses can also remind Plasmodium. To differentiate them from the plasmodium detection helps budding cells.

In cases of dispute, should take into account, malaria parasites that consists of a nucleus and cytoplasm, and mature parasites have also pigment. If you find only the nucleus in a smear or just cytoplasm diagnosis of malaria can not be put.

Some representatives of the class Sporozoa parasites in the intestines of man.

Isospora belli (man) infests the villi of the small intestine, sexual and asexual cycles of development takes place in the gut epithelial cells. It causes a rare human disease - Coccidiosis, in which are found in the feces of the oocysts. They are oval-elongated, Colorless, a dual-shell, length 25 33 m, width 10-19 mm, their cytoplasm is filled with highly refractive light grains. The freshly feces oocysts are spherical shape.

Oocysts found in the feces the end of the disease and even after recovery, therefore, early diagnosis is often not possible.

Blastocystis man - Frequent microorganism in feces. In appearance it resembles some species of amoeba cysts. It is round or oval formation of various sizes (from 5 to 20 m or more). All of the central part is occupied by a large vacuole with a homogeneous, refract light weakly substance (Lugol solution turns yellow), kotoroe surrounded neširokim of opening citoplazmy. In the cytoplasm there is one or more cores. Dividing shapes look like a biscuit. For a man Blastocystis man nonpathogenic, the presence of large amounts of it in the stool is considered an indirect indicator of the state of catarrhal intestinal mucosa.

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