Methods for detection of protozoa in the feces by formalin-ether concentration

When formalin-ether treatment separates the feces and concentrating protozoan cysts, parasites in the guts.

The reagents for the detection of protozoa

• Formalin solution (10 ml concentrated formalin, 0,85 g sodium chloride, to 100 ml of distilled water).
• Ether.
• Lugol solution (stable for months when stored in the dark at room temperature the dishes).

Methods of detecting the simplest method of formalin-ether concentration

The tubes are poured on 6 ml formalin. Feces particle size of a small pea-sized wooden stick (length 100- 150 mm and a thickness of 2-3 mm) introduced into the test tube and thoroughly emulsified in a solution of formalin. Then it was added 2 ml ether, closed with a rubber stopper, vigorously shaken for 1 min and centrifuged 3 minutes at 1500 / min.

After centrifugation, between the layers of formalin and ether layer should be formed stool. Wooden stick it carefully separated from the walls of the tube and poured the entire contents of the tube, maintaining benthic sediment. Holding down the tube hole, quickly wipe it with a cotton swab the inner walls, to remove the greatest possible amount of liquid. Then invert the tube opening up, pipette collect the remaining liquid from the bottom of the test tube, transfer it to slide, and then add a drop of Lugol, covered with a coverslip and examined under a microscope at low and high magnification. If necessary, the emulsion stool can be stored in formalin for one- two days.

Evaluation of the results of research by the formalin-ether concentration

When viewing a note of all the drug Detect. The method allows the addition of vegetative forms and identify simple cysts.