The technique of microscopic examination of urine
Delivered to the lab urine is shaken and poured into a flask tripod, where as soon as it is measured relative density. The flask was allowed to stand for urine 1 no, the precipitate is then collected in a centrifuge tube (half tubes). After centrifugation, the supernatant was not drained, and immediately take the precipitate for microscopy.
If you suspect the presence of tumors in the kidneys or urinary tract after taking urine sediment poured portions into Petri dishes with a thin layer to detect a variety of small pieces, threads, clots, etc.. Cups are considered on a black background. From scraps found a narrow spatula and dissecting needle, preparations for microscopic examination. This simple method of selection macroscopically visible particles is often the only, allows to detect cancer cells in the urine. So no further action, associated with cytological diagnosis of renal tumors or bladder, they can not be neglected.
Successful microscopic examination may only, when the urine is fresh and in a clean container. Before the urine sediment microscopy is necessary to have data of physical and chemical analysis, including information about the presence or absence of protein in the urine, as well as information on age and sex of the patient, suspected diagnosis.
Microscopic examination of the urine helps to establish a violation of kidney function, identify elements, characteristic of inflammation and other pathological processes in the urinary organs. First, the drug is considered a small increase in (eyepiece 8x), and then under high - (40X eyepiece). At low magnification easier to detect cylinders, clusters and groups of erythrocytes and leukocytes, individual crystals and Druze, as well as other elements, found in small quantities in urine.
At high magnification It is clearly visible structure elements of urine, some of them can be found only at this magnification (erythrocytes, devoid of hemoglobin, fragmented erythrocytes, grain lipids from the secretion of the prostate and others.). There are elements of organized and unorganized urine sediment. The main elements are organized sediment erythrocytes, leukocytes, epithelium and cylinders.