Research secretory function of the stomach – Chemical study of stomach contents

Determination of the proteins in the stomach

In some diseases of the stomach in its content significantly increases the number of proteins. Normally it is between 0.06-0.1 up 2,8 g / l.

For studying protein composition of gastric juice used electrophoresis method.

Analyzed gastric juice should not contain impurities as foreign protein, so as a test meal can not use broth or cabbage broth. Not suitable for the study of gastric juice, colored bile, blood or food containing residues. Since the first portion of gastric juice can contain admixture of food debris or saliva, to study and take the next batch right out put them in the refrigerator for termination of proteolytic enzymes in the stomach proteins. Application of electrophoresis allows to allocate fractions 8-10 krupnomolekulyarnyh connections gastric juice.

Distinguish two major pathological type electrophoregrams.

The first type (cathode) characterized by densitometric curve pointed and tapered shape. Observed in chronic gastritis with secretory insufficiency.

The second (anode) type characterized by a stretched densitometric curve. This type of curve occurs with duodenal ulcer with increased secretion of the stomach.

The disadvantage is the inability to study squirrel differentiation krupnomolekulyarnyh sources of these compounds in the gastric juice (only a fraction of them produced the gastric mucosa). The protein composition of the gastric juice is largely dependent on pathological processes, occurring in the body.

Determination of the amount of chlorides, contained in gastric juice

Determination of the amount of chlorides gives some idea of ​​the acid-forming and secretory function of the stomach, the processes of interaction between acidic and alkaline components secret. Determine the chloride method Folgardta.

About secretory function of the stomach can be seen as manifestations of the time in the gastric juice of neutral red, Preset patient (gastrohromoskopiya). In the study of the functional state of the stomach is of secondary importance gastrohromoskopiya.