Lumpy light – status and sputum
Actinomycosis lung characterized by the formation in the lung is very dense infiltrates, prone to soften and the emergence of fistulas, of which is allocated a yellowish pus.
If you collect the discharge of these fistulas in a petri dish and cook thin smear (directly in a cup with a small amount of pus or slide), that the study of it on a dark background can detect very small yellowish or whitish-gray to greenish grains, representing Druze radiant fungus.
For microscopic examination of pus Druze washed and examined in a drop of water or glycerin. At low magnification fully visible Friends typical radiant edge. With the help of color can be detected Tsilyu- Nelsenu flask-shaped thickening at the ends of the mycelium strands. When painting with friends on Gram-positive Gram thread mycelium (dark blue), and flasks - Gram (Pink colour). Sometimes you can find only fragments of mycelium strands. If the study of pus from fistulas mushroom elements can not be detected, resorting to complex research, comprising the culture is in the culture media, histological examination, etc..
Lumpy light study punctate exposed light and phlegm, which are selected from yellowish or grayish-white grains, studied in the native preparation, and then stained with Gram.