Detection of helminth eggs in the stool by enrichment
Cal suspenziruyut in the flotation solution, having a large relative density, than helminth eggs. Wherein helminth eggs float to the surface, the resulting film is examined under a microscope.
IN used as a reagent flotation solution for Kalantaryan (1 kg of sodium nitrite dissolved in 1 l of water, boil the mixture to form a film, and poured without filtration dry bottle; the relative density of the solution 1,38) or flotation solution for Brudastovu - Red Nose (900 g of sodium nitrate, and 400 g of potassium nitrate dissolved during heating in 1 l of water; the relative density of the solution 1,47-1,48).
Methods of detection of helminth eggs in the stool by enrichment
In a beaker thoroughly mixed with a glass rod 5-10 g of feces and 100- 200 One ml solutions of flotation. Immediately after mixing remove glass rod surfaced on the surface of large particles. To the surface applied saline slide. If between the mixture and the slide is a blank space, the saline solution is added to the ground mixture to complete the slide.
Allowed to stand for 20-30 minutes, after which the slide is removed, put under the microscope film up and browsing without cover glass entire film, adhering to the surface of the slide. To avoid drying out during film study can be mixed with a two-- on me to three drops 50 % glycerol solution.
Consider all found in the product helminth eggs.
The described method can detect infection Ascaris, vlasoglavami, ankilostomidami, teniidami, trematodami, tapeworm and other types of worms.
