Method Krasil'nikova – Detection of helminth eggs in the feces using detergents

Under the action of surfactants, included in the detergent, helminth eggs are released from the feces and are concentrated in the sediment.

As the reagent used 1 % a solution of washing powder (powder was dried in an oven at 100 ° C for 1-2 h, 10 g of the powder was dissolved in 1 liter of tap water).

Method 1

In the flask with a capacity of 30-50 ml pour 20-30 ml of detergent, there is placed a portion of feces the size of a hazelnut. Feces desirable for research placed in a detergent solution no later than 1 hours after defecation. The ratio of the solution and feces around 1 : 2. Feces should be in solution at least one day. During this time the vial is formed in the bottom two-, three-layer pellet. The bottom layer consists of coarse hard particles, the middle layer concentrate helminth eggs, which sometimes settle light flakes. Pasteur pipette with a broken high end is introduced into the middle layer of sludge, perhaps lower, but not touching the bottom of the bottle, gaining one to three drops of fluid and transfer them onto a glass slide. A droplet covered with a cover glass plate or cellophane on Kato and examined under microscope. One glass should be cooked two drugs.

Method 2

Feces were placed in a detergent solution at a ratio of approximately 1:10 and stirred to form a suspension. Through 30 min contents of the tube shaken for 1-2 minutes and centrifuged 5 at 1000-1500 m / min. From sediment prepare two preparations on the same slide.

Viewers under the microscope completely both drugs, considering all the helminth eggs found.

The described method can detect all kinds of helminth eggs, allocation with the feces.