Method and Harada Mori – Detection helminth larvae in the feces by culturing them on filter paper

The warm wet filter paper from eggs or hookworm hookworms, allocation with the feces, larvae develop filariform, which can be readily detected.

Methods of detecting larvae of worms in feces by culturing them on filter paper

On a strip of filter paper is applied 150h15 m 0,5 g of feces so, at the ends of the strips remain free. The paper was placed into tubes on 1/4 filled with water, to one end of the strip without feces it was submerged in water, and another appeared from the tube and held cork. The tube for five or six days, was placed in a thermostat at a temperature + 28° C. During warmer months, you can leave the tube at room temperature, increasing the incubation time of 8-10 days.

Filariform larvae, developed from eggs at this time, They fall on the filter paper and are deposited on the bottom of the tube. After incubation the tubes were removed from the paper and destroys, and the fluid is examined under a microscope or with the naked eye at the bottom of the side light tubes. In doubtful cases, the liquid is centrifuged and the precipitate was examined under a microscope.

It must be remembered, cal for research that should be taken no later than 1 hours after defecation. When examined under a microscope or with the naked eye at the bottom of the tube side light reveals filariform American hookworm larvae of hookworms or. This method allows to reveal hookworm infection or American hookworm and differential diagnosis of diseases, they cause.